Journal: Journal of Translational Medicine
Article Title: Hypoxic BMSCs accelerate femur fracture healing via the METTL3/SLC2A3 m6A-glycolysis axis
doi: 10.1186/s12967-025-07510-2
Figure Lengend Snippet: Hypoxia induces higher osteogenic capacity in BMSCs . ( A ) Flow cytometry assesses the expression levels of BMSCs markers (CD29 and CD44) and another cell marker (CD45). ( B ) CCK-8 assay was conducted to assess the cell viability of BMSCs after stimulation with different concentrations of CoCl₂ for 72 h. * P < 0.05, ** P < 0.01, *** P < 0.001, one-way ANOVA. ( C ) Alizarin Red staining detects the osteogenic capacity. ( D ) Immunofluorescence assay showed the expression level of HIF1α. ( E ) ELISA analysis of ALP levels in Ctrl and Hypoxia groups. ( F ) Runx2, OCN, and Osx biomarkers were assessed using WB. *** P < 0.001, Student’s t -test
Article Snippet: BMSC identity was confirmed by flow cytometry (NovoCyte, Agilent, USA) using antibodies against CD29 (No. 130-119-165), CD44 (No. 130-107-802), and CD45 (No. 130-128-256; 1:100 diluted, Miltenyi Biotech, Germany).
Techniques: Flow Cytometry, Expressing, Marker, CCK-8 Assay, Staining, Immunofluorescence, Enzyme-linked Immunosorbent Assay